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KMID : 1177720110050010005
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Journal of Alternatives to Animal Experiments
2011 Volume.5 No. 1 p.5 ~ p.9
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Value of keratinocyte cell Lines to screen skin sensitizing chemicals
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Kim So-Nam
Yeo Kyeong-Uk
Hwang So-Ryeon
Jo Ji-Hoon
Sohn Soo-Jung
Yum Young-Na
Lee Yong-Kyoung
Heo Yong
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Abstract
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Alternative methods using various human or animal cell lines are under development to classify chemical ingredients to skin sensitizing or irritant chemicals. Keratinocyte cell line could be a good target cell, in that the cells are the first contact point for chemical absorption through skin, and immune reactions including inflammation are occurred through interactions among keratinocyte, Langerhans` cell, and T cells. This study introduced current research activities on development of alternative methods using keratinocyte cell lines for identifying skin sensitizers. In addition, a good laboratory practice was introduced to perform serial passage culture of HaCaT human keratinocyte cell line. Furthermore, optimal cell density and culture duration were demonstrated to determine both intracellular and extracellular levels of IL-1α, IL-6, IL-8, and IL-18 from HaCaT cells.
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KEYWORD
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skin sensitization, keratinocyte cell Line, HaCaT, interleukin-18
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